Fig. 8

In addition to TJ disruption leading to BBB damage, decreased Mfsd2a expression after TBI leads to an increase in CAV-1-mediated vesicle transport and is involved in BBB damage. A, B Immunostaining for claudin-5 (green) and CD31-positive endothelial capillary profiles (red) in brain slices; nuclei were stained with DAPI. scale bar = 100 μm (A). The corresponding statistics are shown in (B). n = 4/group. C, D, E, F The protein levels of claudin-5 (D), caveolin-1 (E) and albumin (F) in pericontusional brain tissues on day 3 after TBI were measured by WB. n = 4/group. G, H Representative images (G) and quantitative analyses (H) of the degree of EB dye extravasation in the ipsilateral cortices of each group on day 3 after TBI. n = 6/group. I The brain water content in each group on day 3 after TBI. n = 10/group. All data was analyzed by one-way ANOVA and Tukey’s multiple comparisons test, all values are presented as the mean ± SD. (-): TBI only; (Ctr): control shRNA; (Vec): vehicle; (OE): overexpression of Mfsd2a