Fig. 1

Isolation of cerebral cortical capillary and parenchyma by LMD and the validation of the purity of the isolated capillaries. a Aβ immunostaining was performed using the FFPE sections from ADNC +/CAA +, ADNC +/CAA −, and ADNC −/CAA − groups as previously described [5]. Two photographs in different regions are shown for each group. Black arrows indicate the cerebral vessels in occipital cortex. White arrows indicate the Aβ deposits in cortical parenchyma (except for vessels). Scale bar, 20 µm. All vessels in cortical regions were covered with Aβ in ADNC +/CAA + sections. For each group, the neighboring sections were used for the proteomic analysis (eosin staining). b Photographs of eosin staining before and after LMD. Scale bar, 50 µm. The eosin staining enabled to identify the cerebral vessels and cortex regions in the FFPE sections. The vessels (except for large vessels) and parenchyma in cortex were isolated by LMD as shown in the photographs until the total dissected area reaches 15 mm² (× 20 μm thickness = 0.3 mm³) for each sample. c Relative protein expression levels of two endothelial cell markers in the collected capillaries and parenchyma. The expression level of each protein was normalized by the average of the protein expression level in ADNC −/CAA − capillaries as described in the "Materials and methods" section. Black plot, ADNC +/CAA +; Gray plot, ADNC +/CAA −; White plot, ADNC −/CAA −. Circle, capillary samples; Diamond, parenchymal samples. **BH-adjusted p < 0.01, significantly different between two groups. NS not significantly different (BH-adjusted p > 0.01)