Fig. 6

AZE’s effect on the activity of selected key CSF secreting transporters. ⁸⁶Rb⁺ efflux rate (a read out for NKCC1 activity) is presented as a function of time upon treatment with either 20 µM bumetanide (A) or 200 µM AZE (C). The efflux rate constant was quantified using linear regression analysis for bumetanide (B) and AZE (D). ⁸⁶Rb⁺ uptake rate (read out for Na⁺/K⁺-ATPase activity) is shown in counts per minute (cpm) after treatment with 2 mM ouabain (E) and after treatment with 200 µM AZE (F). Na⁺ _i sensitive SBFI dye traces during exposure to 200 µM AZE or control solution are shown in G, and quantified in H (CTRL: -0.7 ± 3.0%, AZE: 1.2 ± 3.7%, n = 6, P = 0.34). All experiments performed on choroid plexus isolated from 6 animals (n = 6) and unpaired t-test used to assess the significance. CTRL – control, AZE – acetazolamide, BUM – bumetanide, OUA – ouabain. Data are shown as mean ± SD. **; P < 0.01, ***; P < 0.001, ns; not significant