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Fig. 5 | Fluids and Barriers of the CNS

Fig. 5

From: Acetazolamide modulates intracranial pressure directly by its action on the cerebrospinal fluid secretion apparatus

Fig. 5

Live imaging of fluorescent dye as a proxy of CSF secretion following treatment with AZE. The top panel in A illustrates pseudo-color fluorescence superimposed on a white light image from a rat after ventricular injection of IRDye 800 CW carboxylate dye. The white box below lambda (skull landmark) illustrates the area for dye intensity quantification. The bottom panel illustrates representative images of fluorescence signal 0.5 and 5 min after i.c.v. injection of control solution or 2 mM AZE solutions (expected ventricular concentration 200 µM, see Methods). Quantification of the dye flow expressed as arbitrary units (a.u.) normalized to the first image after i.c.v. injection is shown in panel B (n = 4, P < 0.05, unpaired t-test). Panel C shows the quantification of the dye flow normalized to the first image after i.v. injection of control solution or 100 mg kg−1 AZE (nCTRL = 6, nAZE = 4, P < 0.05, unpaired t-test). CTRL – control, AZE – acetazolamide. Data are shown as mean ± SD. *; P < 0.05, ns; not significant.

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