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Fig. 5 | Fluids and Barriers of the CNS

Fig. 5

From: Brain microvascular endothelial cell dysfunction in an isogenic juvenile iPSC model of Huntington’s disease

Fig. 5

Tissue-engineered BBB microvessel model incorporating HD180 or HD-corrected iBMECs. A Schematic illustration of fabrication of three-dimensional microvessels seeded with iBMECs; (left) front view, (right) side view. B Microvessels with HD-corrected and HD180 iBMECs similarly restrict Lucifer yellow transport. Representative images are shown. C Lucifer yellow permeability in 3D microvessels. Data collected across n = 5 (HD-corrected) and 4 (HD180 iBMECs) independent differentiations. D Turnover rates of microvessels. Data collected across n = 5 (HD-corrected) and 4 (HD180 iBMECs) independent differentiations. E, F Adhesion of monocyte-like cells to tissue-engineered microvessels: E Data collected across n = 4 (HD-corrected) and 3 (HD180) independent differentiations of iBMECs seeded into microvessels. F Representative images of adherent cells after washout. THP-1 fluorescence is oversaturated to assist in visualization

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Fluids and Barriers of the CNS

ISSN: 2045-8118