Fig. 5
OVX on 55Fe-Tf brain uptake in females. Samples are reported as DPM per gram of brain tissue. After removing circulating estrogen via OVX, female mice still do not show changes in 55Fe-Tf uptake (A), release (B), or total uptake (C) across infusion conditions. Intact sham mice were included as a control. The coefficient of variability of 55Fe-Tf uptake in sham conditions was 16.15%. The levels of plasma estradiol levels were determined to confirm the success of the OVX in all mice used (D). The TIBC of plasma is higher in OVX mice while the Tf saturation percentage and serum iron are lower when compared to control (E). n= 5 for all conditions, means of biological replicates ± SD were evaluated for statistical significance using one-way ANOVA with Tukey’s posttest for significance for A and B. Means ± SD were evaluated for statistical significance using unpaired t-test for significance for C and D. **** p<0.0001