Fig. 3

⁵⁵Fe-Fth1 brain uptake in males and females. Samples are reported as DPM per gram of brain tissue. In males, the ratio of apo- to holo-Tf in the brain has little regulatory effect on uptake into MVs (A), release into the parenchyma (B), or uptake into the whole brain (C). In females, the ratio of apo- to holo-Tf in the brain has little regulatory effect on uptake into MVs (D) or whole brain (F) but increased ratio of apo-Tf increases release into the brain (E). Notably, the MVs contain substantially more ⁵⁵Fe than the entirety of the brain, supporting the role of MVs in regulating iron release. n = 5 to 6 for all conditions, means of biological replicates ± SD were evaluated for statistical significance using one-way ANOVA with Tukey’s posttest for significance