Fig. 3

Expression of ACE2 around cerebral ventricles. A: Schematic elucidation of the cerebral ventricular system. B–E: Co-staining of ACE2 (green) with astrocyte marker GFAP (red) or neuron marker NeuN (red) in the subventricular zone (SVZ) of the LV, respectively. B–C show the low magnitude images. D–E show the enlarged images of the indicated regions in B and C, respectively. Arrow: indicates astrocyte, Dash arrow: indicates neuron. Arrowhead: indicates microvessel. F: ACE2 (green) expresses in the GFAP-positive (red) and Nestin-positive (red) radial glial cells in the ventricular zone (VZ) of the LV. Arrow: indicates GFAP-positive radial glial cells. Dash arrow: indicates Nestin-positive radial glial cells. Arrowhead: indicates microvessels. G–J: Co-staining of ACE2 (green) with GFAP (red) or NeuN (red) in the 3 V, respectively. G-H show the low magnitude images. I-J show the enlarged images of the indicated regions in G and H, respectively. Arrow: indicates tanycyte, Dash arrow: indicates neuron. Arrowhead: indicates microvessel. L–O: Co-staining of ACE2 (green) and GFAP (red) in the AQ and 4 V, respectively. L–M show the low magnitude images. N–O show the enlarged images of the indicated regions in L and M, respectively. Arrow: indicates astrocyte, Arrowhead: indicates microvessel. P–R: Co-staining of ACE2 (red) with ChP epithelial cell marker claudin-3 (CLN3, green) and ChP capillary endothelial cell marker CD31 (green), respectively. P: Schematic elucidation of the structure of ChP in a cerebral ventricle. Q: ACE2 expresses in the ChP epithelial cells in the 3 V. Qii-iv: the enlarged images of the indicated region in Qi. R: ACE2 expresses in the ChP capillaries. Rii-iv: the enlarged images of the indicated region in Ri. DAPI (blue) was used for nuclear staining. ChP: choroid plexus, Epen: ependymal cell of the ventricular wall, Epi: epithelial cell in the ChP, Endo: endothelial cell in the ChP capillaries. The dash lines in all images outline the border of ventricular cavity. Image magnitude B–C, G–H, L–M, Qi and Ri: 20X, D–F, I–J, N–O, Qii-iv and Rii-iv: 100X