Fig. 6

A After 6 h OGD treatment, pericytes significantly increased COX2 expression. B and C A monolayer or co-cultured BBB model was treated with thromboxane A2 receptor (TP) agonist (U-46619, 20 μM) for 18 h. Barrier function was investigated by TEER. There was no significant difference between control and U-46619 in monolayer model. In contrast, U-46619 decreased the TEER in co-cultured BBB model. D Fasudil (1 μM) improved the barrier dysfunction induced by U-46619. a, b, c, d two-way Anova with Bonfferroni post-test, n = 3, *p < 0.05; ***p < 0.001. d Unpaired t-test, n = 3, ##p < 0.01. All data were presented as mean ± SEM