Fig. 5

A Effect of 24 h fasudil (10 µM) treatment in normoxia and oxygen–glucose deprivation/reperfusion (OGD/R) conditions on cell viability performed by the cell counting kit-8 WST-8 based assay test on primary rat brain endothelial cells kept in monoculture BBB model. Unpaired t-test and two-way Anova with Bonfferroni post-test, n = 5, *p < 0.001; #p < 0.05. Data is presented as mean ± SEM. Effects of fasudil on expression of occludin (B) and claudin-5 (C) in a triple co-culture model with primary brain pericytes and astrocytes under normoxia or OGD/R condition. Immunostaining showed that the expression of occludin and claudin-5 were disrupted in RBEC in the BBB model with OGD/R compared with normoxic controls. Arrows indicate the changes in occludin and claudin-5 distribution. Moreover, fasudil improved the disruption of occludin and claudin-5 under OGD/R. Bar: 50 µm