Fig. 4

Effect of 24 h fasudil (10 µM) treatment in normoxia and oxygen–glucose deprivation/reperfusion (OGD/R) conditions on barrier properties of pimary rat brain endothelial cells kept in monoculture (a and c) or in a triple co-culture model with primary brain pericytes and astrocytes (b and d). Fasudil was given at the beginning of the 24 h reoxygenation. A and B Transendothelial electrical resistance (TEER) measurement values after treatment. C and D Sodium fluorescein (Na-F) permeability after fasudil treatment. Fasudil is protective against OGD/R conditions restoring barrier properties to the level of normoxia control almost in all conditions. n = 3–4, one-way Anova with Bonfferroni post-test, *p < 0.05; ***p < 0.001. All data are presented as mean ± SEM