Fig. 3

Effect of 24 h fasudil (1 and 10 µM) treatment in normoxia on junctional protein expression of primary rat brain endothelial cells kept in a triple co-culture model with primary brain pericytes and astrocytes. A Representative Western blot bands of chosen junctional proteins with the loading control β-actin. B Protein expression calculated from the intensity of the Western blot bands. ZO-1: zonula occludens-1. n = 4, Two-way Anova with Bonfferroni post-test, ***p < 0.001. All data are presented as mean ± SEM