Fig. 2
From: Transcriptional profiling of transport mechanisms and regulatory pathways in rat choroid plexus
Choroid plexus transcriptomic profile is similar to FACS choroid plexus epithelial cells. a–d FACS with fluorescence emission intensity (y-axis) and forward scatter (FSC) (x-axis). a The cell suspension obtained by FACS with primary anti-NKCC1 antibody and secondary antibody, b in the absence of antibody inclusion in the FACS procedure, or c in the absence of primary antibody, but with inclusion of secondary antibody. The target area of capture (cells with high fluorescence emission intensity and large cell size) is defined in a black square in the top right corner (a–c). d Immunohistochemical staining of captured cells with anti-AQP1 (green), marker of F-actin; phalloidin (red), and nucleus marker; DAPI (blue). e–g Depiction of the shared transcribed genes in the pure fraction of choroid plexus epithelial cells versus the whole choroid plexus, e for all genes, f for genes encoding membrane transporters and pumps, and g for genes encoding membrane water and ion channels. Shared genes (in percentages and number) in the sphere center, with the number of non-shared genes depicted on either side