Fig. 2

Transport of α-syn monomers through the BBB model. Representative confocal micrographs of α-syn monomer added to pBECs seeded on filters. Green are stains for the α-syn monomer and blue are Hoechst stains to mark the nucleus of the cells. a 10 min luminal side (b) 10 min abluminal side (c) 2 h luminal side and (d) 2 h abluminal side, scale bars show 15 µm. e Experimental set up: 100 nM α-syn monomer was added on to the BBB cell model and incubated for 10 min or 2 h at 37 °C with shaking at 150 rpm (f) ELISA results for α-syn transport across BBB when added from the luminal or abluminal side. Significance was tested using One-way ANOVA with Šidák´s multiple comparisons test