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Fig. 4 | Fluids and Barriers of the CNS

Fig. 4

From: A novel chronic dural port platform for continuous collection of cerebrospinal fluid and intrathecal drug delivery in free-moving mice

Fig. 4

Monitoring of real-time changes in CSF glucose concentration in the free-moving mice. a Schematic of continuous CSF collection via CDP for monitoring glucose and lactate levels during glucose loading. CSF levels of glucose and lactate were continuously and quantitatively measured using biosensors attached to the middle of the collection tubing (biosensor port). CSF was collected at the flow rate of 0.05 µL/min using a syringe pump. Mice were intraperitoneally injected glucose (2 mg/g body weight) during continuous CSF collection. b Schematic of biosensor port. Two electrodes of the glucose and lactate biosensors are installed within the port. Electrodes are exposed to the continuous flow of CSF and provide real-time changes in glucose and lactate concentrations. c CSF levels of glucose (blue line) and lactate (orange line) after intraperitoneal glucose loading. A representative data is shown from a wild-type mouse. CDP chronic dural port, CSF cerebrospinal fluid, i.p. intraperitoneal injection

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