Processing mechanism of guanidinoacetate in choroid plexus epithelial cells: conversion of guanidinoacetate to creatine via guanidinoacetate N-methyltransferase and monocarboxylate transporter 12-mediated creatine release into the CSF

Table 1 Primers used for gene expression analysis

Genes	Gene bank accession number	Orientation	Primer sequence (5′–3′)	Product size
MCT12	NM_001191637.1	Forward	AGCCTTCCTTCTTTGTGG	179 bp
MCT12	NM_001191637.1	Reverse	TCTGATCTAACTCCTTCGC	179 bp
GAMT	NM_012793.2	Forward	TCTGACACGCACCTGCAGATCC	584 bp
GAMT	NM_012793.2	Reverse	GCATAGTAGCGGCAGTCGGCTG	584 bp
β-Actin	NM_031144.3	Forward	TCATGAAGTGTGACGTTGACATCCGT	285 bp
β-Actin	NM_031144.3	Reverse	CCTAGAAGCATTTGCGGTGCACGATG	285 bp

ISSN: 2045-8118