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Fig. 3 | Fluids and Barriers of the CNS

Fig. 3

From: Effects of acute and chronic oxidative stress on the blood–brain barrier in 2D and 3D in vitro models

Fig. 3

Response to chronic and acute oxidative stress in a tissue-engineered iBMEC microvessel. a Phase contrast and fluorescence images of a control microvessel (treated with vehicle) during 1 h perfusion with tagged fluorescently-labeled 10 kDa dextran. b Heatmap demonstrating the influence of ROCK inhibitor (RI) on TEER in 2D studies. The paired conditions demonstrate that RI sensitizes iBMEC monolayers to oxidative stress. Green asterisks indicate selected conditions for acute and chronic exposure in the presence of RI to further explore in 3D microvessels. Each cell represents the mean of three technical replicates across n = 3 biological replicates in all conditions. c Representative phase and phase/fluorescence overlays during perfusion with 10 kDa dextran at chronic and acute doses of H2O2 showing maintenance of monolayer confluency and dye restriction

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Fluids and Barriers of the CNS

ISSN: 2045-8118