Fig. 5
From: Exploring ITM2A as a new potential target for brain delivery
ITM2A transport assays. A In vitro human brain transcytosis model from BrainPlotting. Papp antibody anti- ITM2A in blue and Papp antibody anti-TFRC in yellow normalized to Papp Control mouse IgG. Results are expressed in Papp ratio (target/control) means ± Standard Deviation p values were obtained by Mann–Whitney test *p = 0.0444. B In vitro human brain transcytosis controls were Papp control antibody, Papp Fluoresceine and TEER measurements in each Transwell. Results are expressed in means ± Standard Deviation p values were obtained by Mann–Whitney test NS: p > 0.9999. C In vivo mouse brain exposure of an ITM2A monoclonal antibody. 5 mg/kg iv injection of Yu093-G04 and anti-TNP to C57Bl6 mice; samples were collected 5 h after injection. Results are expressed as mean ± S.D. (1 experiment, n = 3 animals). p values were obtained by Two-way ANOVA with Sidak’s multiple comparisons test ns: no statistical significance = p > 0.05, ****p < 0.0001