Fig. 4

ITM2A RNA and protein levels. A Relative quantification of ITM2A protein expression in different cells by Western Blot. Green: protein at 60 kDa (ITM2A + GFP), pink: protein at 30 kDa (ITM2A). Western Blot membranes are in Additional file 2. B Quantification of ITM2A in samples of human brain microvessels by LC/MS. ITM2A in green and TFRC in red are expressed as mean ± S.D. (4 quantified peptides for ITM2A and 5 quantified peptides for hTFRC). C Relative quantification of ITM2A protein expression in newborn mice by Western Blot. HEK293 mouse ITM2A GFP was used as control. Green: protein at 60 kDa (ITM2A + GFP), pink: protein at 30 kDa (ITM2A). Western Blot membranes in Additional file 3. D itm2a mRNA relative quantity (RQ) in new-born and adult mouse brain homogenates. Housekeeping gene was gapdh. Results are expressed as mean ± S.D. (n = 1 experiment, performed in triplicates). p values were obtained by Ordinary One-way ANOVA with Dunnett’s multiple comparisons test vs P1. E Quantification of ITM2A in samples of mouse cells and tissues by LC/MS. Cortex in purple, muscles in blue and cells in green. Results are expressed as mean ± SD. (3 quantified peptides)