Fig. 3
From: Exploring ITM2A as a new potential target for brain delivery
ITM2A mRNA and protein detection in endothelial cells from different species. A ITM2A mRNA detection in monkey endothelial cells from cortex analyzed with RNA-seq at different times of culture. P0D0 (blue) = fresh microvessels, P0D7 (green) = endothelial cells after 1 week in culture, P1D7 (magenta) = endothelial cells after 1 thawing then 1 week in culture. B Differences in the mRNA levels of Itm2a (orange) and CD31 (blue) in mouse endothelial bEnd.3 cells. Microvessels named P0D0, primary cells selected by puromycin named P0D7, primary cells selected by CD31 + named P0D0 CD31 + selected and P0D7 CD31 + selected. Actin was used as a housekeeping gene. C Immunofluorescence staining of mouse ITM2A protein in rodent endothelial cells with three ITM2A antibodies. D Immunofluorescence staining of hCMEC/D3 with TFRC and ITM2A antibodies. Labelling is visualized with secondary anti-species Alexa 647 antibodies in red. Nuclei are labelled with Hoechst in blue