Fig. 1

Validation of ITM2A as a potential brain delivery target and stable cell line generation. A Flowchart, go steps are highlighted with blue arrow, no-go steps are highlighted with red arrow. B Human or mouse tagged ITM2A plasmid design. Each construct has intracellular domain (ICD) in blue, transmembrane domain (TM) in yellow and extracellular domain (ECD) in green with Brichos domain; HA tag localization in red, GFP tag in fluo green. C Human or mouse GFP tagged ITM2A expression in HEK293 cells; HEK293 cells are stably recombined with human or mouse ITM2A with a GFP tag at different positions; GFP-tagged ITM2A is visualized in green, and nuclei are stained in blue by Hoechst. D Highlighting the ITM2A colocalization in HEK293 human ITM2A N-Ter GFP cell compartments. Confocal images of N-Ter GFP ITM2A in green and anti-Giantin antibody (ab37266) in red or anti-LAMP1 antibody (ab24170) in red. Yellow color indicates colocalization in the same focal plan, red and green colors indicate no colocalization in the same focal plan