Fig. 5From: Acquired hydrocephalus is associated with neuroinflammation, progenitor loss, and cellular changes in the subventricular zone and periventricular white matterSubventricular zone alteration in the hydrocephalic pigs. The cell densities of A proliferative cells (PCNA+), B PCNA+ with GFAP+ , and C βIII tubulin + Ki67 + cells in the ASVZ were reduced in the hydrocephalic (hydro) pigs compared with the sham control (sham) pigs. Representative immunofluorescence for PCNA (red) in sections of the ASVZ from D a sham control and E a hydrocephalic pig illustrating the decrease of proliferative cells. Magnifications are the same. Separated channels for fluorescence for (F–F′′′) PCNA (red) and GFAP (green), and (G–G′′′) βIII tubulin (red) and Ki67 (green) with same magnifications. H Oligodendrocytes (Olig2, green) were also reduced in the ASVZ of J hydrocephalic pigs compared with the I sham control. Nuclear staining in blue with DAPI. Scale bars represent 15 µm in D, E; 15 µm in F–F′′′; 12 µm in G–G′′′, 30 µm in I, J. Images were obtained under the confocal microscope with 1 µm thickness. **p = 0.0047 for PCNA, **p = 0.0082 for PCNA + GFAP, **p = 0.0087 for βIII tubulin + Ki67, **p = 0.0095 for Olig2, Wilcoxon–Mann–Whitney testBack to article page