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Fig. 8 | Fluids and Barriers of the CNS

Fig. 8

From: Blood–brain barrier dysfunction in l-ornithine induced acute pancreatitis in rats and the direct effect of l-ornithine on cultured brain endothelial cells

Fig. 8

a Transmission electron micrographs of primary rat brain endothelial cells kept in a triple BBB co-culture model. Effect of l-ornithine (l-orn, 20 mM, 24 h) treatment was investigated on the ultrastructural changes. The negatively charged surface glycocalyx was labeled with the cationic dye Alcian blue. ER: endoplasmic reticulum, L: luminal side, m: mitochondria, n: nucleus, TJ: intercellular tight junction. Black arrows in the l-ornithine treated group point to plasma membrane deformities. Asterisks show irregular glycocalyx staining by Alcian blue. Bar: 1 µm. b Effect of direct l-ornithine (l-orn, 20 mM) treatment on the surface charge of cultured rat primary brain endothelial cells. Values presented are means ± SEM. n = 3–10 with a total of 15–111 rounds of measurements. Statistical analysis: one-way Anova followed by Bonferroni post-test, ***, p < 0.001

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