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Fig. 6 | Fluids and Barriers of the CNS

Fig. 6

From: Blood–brain barrier dysfunction in l-ornithine induced acute pancreatitis in rats and the direct effect of l-ornithine on cultured brain endothelial cells

Fig. 6

Effects of l-ornithine (l-orn, 20 mM, 24 h) on junctional morphology and adhesion molecule expression. a Representative immunofluorescent pictures of transmemebrane tight junction proteins claudin-5 (Cl-5) and occludin (Occl), junctional associated molecules zonula occludens-1 (ZO-1) and β-catenin (β-cat), and cell adhesion molecules intercellular adhesion molecule-1 (Icam-1) and vascular cell adhesion molecule-1 (Vcam-1). Arrows point to membrane perturbations, stars indicate monolayer discontinuity. C: control. Scale bar: 20 µm for junctional stainings and 25 µm for Icam-1/Vcam-1. b Ratio of the junctional and the cytoplasmic immunostaining intensities. Random pictures (4–5 images/sample), from 2–3 different experiments (n = 15–25) were compared. c Fluorescence intensity of cell adhesion molecules Icam-1 and Vcam-1. Random pictures (4–5 images/sample), from 2 different experiments (n = 18–28) were evaluated. Values presented are means ± SEM, statistical analysis: unpaired t-test. *, p < 0.05; ***, p < 0.001

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