Fig. 6

Modeling neuron-endothelial interactions in Alzheimer’s disease using iBECs treated with conditioned media (CM) from hiPSC-derived neurons. a Schematic depicting CM treatments. Created with Biorender.com. b–e On day 2 or day 9, transwells were organized into treatment groups (APP^WT and APP^Swe/+) such that TEER means were approximately equal and treated with hiPSC-neuronal CM. The CM effect on iBEC TEER was measured after 24 h treatment, on day 3 or day 10, respectively. Two independent differentiations of iBECs (Exp I and II) were conducted to test conditioned media from one differentiation of hiPSC-neurons (n = 3–4 transwells per group). Results were confirmed with one additional differentiation of iBECs (Exp III) treated with CM from another differentiation of hiPSC-neurons (n = 4–5 transwells per group). TEER values were normalized to APP^WT control. Values used to normalize results (in \(\Omega\)*cm²) are 5228.93 (Exp I Day 2), 4134.45 (Exp II Day 2), 5837.78 (Exp III Day 2), 3967.91 (Exp I Day 3), 2888.06 (Exp II Day 3), 4544.19 (Exp III Day 3), 2567.82 (Exp I Day 9), 5497.35 (Exp II Day 9), 4288.35 (Exp III Day 9), 1989.64 (Exp I Day 10), 3448.05 (Exp II Day 10), 3709.38 (Exp III Day 10). ***p < 0.001 (Unpaired two-tailed t-test). f–g Quantification of A \(\beta\)-40 and -42 in APP^WT and APP^Swe/+ conditioned mediums (CM). Four independent differentiations of hiPSC-neurons were performed with n = 2 wells quantified per differentiation. b–g Means are displayed with their SE