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Fig. 5 | Fluids and Barriers of the CNS

Fig. 5

From: Prolonged culturing of iPSC-derived brain endothelial-like cells is associated with quiescence, downregulation of glycolysis, and resistance to disruption by an Alzheimer’s brain milieu

Fig. 5

Effects of TNF-⍺ treatment on TEER. a–d On day 2 or day 9, transwells were organized into treatment groups such that TEER means were approximately equal and treated both luminally and abluminally with 100 ng/ml TNF-⍺ or PBS vehicle (Veh) control. TEER was measured after 16 h treatment, on day 3 or day 10, respectively. Two independent differentiations of iBECs (Exp I and II) were performed with n = 5–7 transwells per group. TEER values were normalized to Veh control. Values used to normalize results (in \(\Omega\)*cm2) are 3315.64 (Exp I Day 2), 3946.00 (Exp II Day 2), 2956.04 (Exp I Day 3), 2634.52 (Exp II Day 3), 4089.16 (Exp I Day 9), 2859.42 (Exp II Day 9), 4546.61 (Exp I Day 10), 3291.39 (Exp II Day 10). ***p < 0.001 (Unpaired two-tailed t-test). Means are displayed with their SE

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Fluids and Barriers of the CNS

ISSN: 2045-8118