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Fig. 4 | Fluids and Barriers of the CNS

Fig. 4

From: Prolonged culturing of iPSC-derived brain endothelial-like cells is associated with quiescence, downregulation of glycolysis, and resistance to disruption by an Alzheimer’s brain milieu

Fig. 4

Glucose transport kinetics in GM25256 iBECs. a Comparison of luminal-to-abluminal glucose transport saturability kinetics on days 2 and 9 after subculture. Glucose transport rates (14C-DG Pe) were compared in the presence (55.5 mM Glucose, green) or absence (5.5 mM Glucose, black) of excess glucose in the luminal compartment to examine glucose transport system saturability. Three independent differentiations were performed to with n = 4–5 transwells per group. b Comparison of abluminal-to-luminal glucose transport saturability kinetics on days 2 and 9 after subculture. 14C-DG Pe values were compared in the presence (55.5 mM Glucose, green) or absence (5.5 mM Glucose, black) of excess glucose in the abluminal compartment to examine glucose transport system saturability. One differentiation was performed with n = 5 transwells per group. ***/###p < 0.001 (Two-way ANOVA with Tukey’s multiple comparisons test). a–b 14C-DG Pe values were normalized to the unsaturated Pe on day 2. c Luminal-to-abluminal 14C-DG Pe on day 9 after subculture were compared with (blue) and without (black) continual media changes on days 4, 6, and 8. One differentiation was performed with n = 6 transwells per group. (Unpaired two-tailed t-test). a–c Means are displayed with their SE

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Fluids and Barriers of the CNS

ISSN: 2045-8118