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Fig. 3 | Fluids and Barriers of the CNS

Fig. 3

From: Prolonged culturing of iPSC-derived brain endothelial-like cells is associated with quiescence, downregulation of glycolysis, and resistance to disruption by an Alzheimer’s brain milieu

Fig. 3

Effects of length of subculture and medium changes (MC) on iBEC expression of glucose metabolism/transport-related proteins. a Representative immunoblots of PFKFB3, GLUT1, HK2, and MCT1 expression on days 2 and 9 after subculture with no MC (black) vs. on day 9 after continual MC on days 4, 6, and 8 (blue). b–e Quantification of immunoblots. Expression was calculated relative to \(\beta\)-actin and normalized to day 2. Each data point represents one well. For PFKFB3 and GLUT1, two independent differentiations were performed with n = 3 wells per group to evaluate expression of days 2 and 9 after subculture with no media changes. Then, two more independent differentiations were performed with n = 3 wells per group to additionally evaluate expression on day 9 with continual media changes on days 4, 6, and 8. For HK2 and MCT1, two independent differentiations were performed which evaluated all three groups with n = 3 wells per group. *p < 0.05, ***p < 0.001 (vs. day 2); ###p < 0.001 (One-way ANOVA with Tukey’s multiple comparisons test). Means are displayed with their SE

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Fluids and Barriers of the CNS

ISSN: 2045-8118