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Fig. 2 | Fluids and Barriers of the CNS

Fig. 2

From: Prolonged culturing of iPSC-derived brain endothelial-like cells is associated with quiescence, downregulation of glycolysis, and resistance to disruption by an Alzheimer’s brain milieu

Fig. 2

Immunocytochemistry and immunofluorescent analyses of TJP and AJP in GM25256 iBECs. a Representative immunocytochemistry of CLDN5 (green), OCLN (green), ZO-1 (red), PECAM-1 (green), and VE-Cad (red) on days 2 and 9 after subculture, counterstained with DAPI (blue). b–f Immunofluorescence analyses of CLDN5, OCLN, ZO-1, PECAM-1, and VE-Cad mean fluorescence intensities (MFI) relative to DAPI MFI on days 2 and 9 after subculture (normalized to day 2). Each data point represents the average of 3–4 random fields of view per well. Two independent differentiations were performed with n = 3 wells imaged per differentiation. **p < 0.01, ***p < 0.001 (Unpaired two-tailed t-test). Means are displayed with their SE. g Representative immunoblots and immunoblot quantification of CLDN5 and OCLN expression on days 2 and 9 after subculture. Expression was calculated relative to \(\beta\)-actin and normalized to day 2. Each data point represents one well. Two independent differentiations were performed with n = 3 wells for each differentiation. ***p < 0.001 (Unpaired two-tailed t-test). Means are displayed with their SE. h TEER trajectories of GM25256 iBECs. TEER was evaluated on days 2 and 9 after subculture. Each line represents an independent differentiation of iBECs (n > 10 for all differentiations). Means of technical replicates are displayed

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Fluids and Barriers of the CNS

ISSN: 2045-8118