Table 1 Overview of primers and conditions. *C.s.: Cre-ERT2 specific primers
Genotype | Primer sequence | Primer conc | Annealing temp |
|---|---|---|---|
HIF-1α | Fw. 5’-GCAGTTAAGAGCACTAGTTG-3’ Rv. 5’-GGAGCTATCTCTCTAGACC-3’ | 400 nM 400 nM | 57 °C |
SMMHC | Fw. 5’-TGACCCCATCTCTTCACTCC-3’ Rv. 5’-AACTCCACGACCACCTCATC-3’ *C.s. 5’-AGTCCCTCACATCCTCAGGTT-3’ | 600 nM 300 nM 600 nM | 58 °C |
GFAP | Fw. 5’-CCTGGAAAATGCTTCTGTCCG-3’ Rv. 5’-CAGGGTGTTATAAGCAATCCC-3’ | 200 nM 200 nM | 59 °C |
GLAST | Fw. 5’-GAGGCACTTGGCTAGGCTCTGAGGA-3’ Rw. 5’-GAGGAGATCCTGACCGATCAGTTGG-3’ *C.s. 5’-GGTGTACGGTCAGTAAATTGGACAT-3’ | 400 nM 400 nM 400 nM | 58 °C |