Fig. 7
HIF-1 deletion prevents hypoxic barrier disruption without altering VEGF or TGF-β levels in brain tissue or serum. VEGF and TGF-β levels measured by ELISA in brain cortex protein lysates (pg/mg normalized to total protein content, panels A, B) and serum (pg/ml, panels C, D) of SMMHC-CreERT2; HIF-1αfl/fl mice treated with (LoF) or without (Ctrl) tamoxifen after 96 h normoxia (Nx, 21% O2) or hypoxia (Hx, 8% O2). Two-way ANOVA, mean ± SD, n = 3 **p < 0.01 compared to Nx Ctrl. Two-way ANOVA, mean ± SD, n = 5, *p < 0.05, **p < 0.01, ***p < 0.001 compared to Nx Ctrl and Nx LoF