Fig. 4
Pericyte-targeted HIF-1 deletion prevents hypoxia-induced vessel dilation. A Representative images of brain microvessels stained with CD31 (green) in cortex, subcortex and hippocampal regions of SMMHC-CreERT2; HIF-1αfl/fl mice treated with oil (Ctrl) or tamoxifen (LoF) after 96 h normoxia (Nx, 21% O2) or hypoxia (Hx, 8% O2). Scale bar = 50 µm. Quantification of mean vessel diameter (B), number of vessels (C) and vessel density (D) in brain cortical regions during normoxia and hypoxia in Ctrl and LoF mice. Two-way ANOVA, mean ± SD, n = 3, *p < 0.05, **p < 0.01, compared to Nx Ctrl, δp < 0.05, compared to Hx Ctrl