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Fig. 8 | Fluids and Barriers of the CNS

Fig. 8

From: Hypoxia increases expression of selected blood–brain barrier transporters GLUT-1, P-gp, SLC7A5 and TFRC, while maintaining barrier integrity, in brain capillary endothelial monolayers

Fig. 8

Transendothelial electrical resistance (TEER) measurements, mannitol permeability and immunocytochemical characterization of confluent brain capillary endothelial cells (BCECs) exposed to hypoxia. A TEER was measured across cell monolayers from the Experimental Day (day 6), where cells were exposed to hypoxia (grey bar) or normoxia/control (white bars) and for 48 h. Measurements were normalized to normoxic control (0 h time point). Data are shown as mean ± SD of three individual batches of triplicates (n = 3, N = 3). B The mRNA expression level of CLDN5, TJP1 (ZO-1), SLC2A1 (GLUT-1), TFRC and ABCB1 (P-gp) in confluent BCECs exposed to hypoxia (grey bars) or normoxia (white bars). Expression levels were normalized relative to normoxic control at time point 0 h. YHWAZ, SDHA, and HPRT-1 were used as reference genes. Data are shown as mean ± SD of three individual batches of triplicates (n = 3, N = 3). C Immunocytochemical characterization of confluent BCEC monolayer exposed to hypoxia or normoxia for 48 h. Control indicates cells at time point 0 h. The cell monolayers were immunostained for claudin-5 (Cl-5), zonula occludens-1 (ZO-1), P-glycoprotein (P-gp) and GLUT-1. All samples were counterstained with propidium iodide (red) to visualize cell nuclei. Scale bars = 10 μm. Images are representative of three individual experiments in triplicate (n = 3, N = 3)

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Fluids and Barriers of the CNS

ISSN: 2045-8118