Fig. 7From: Hypoxia increases expression of selected blood–brain barrier transporters GLUT-1, P-gp, SLC7A5 and TFRC, while maintaining barrier integrity, in brain capillary endothelial monolayersImmunocytochemical characterization of BCEC monolayers cultured under normoxic conditions in the absence or presence of 10 μM DFO and under hypoxic conditions. The cell monolayers were immunostained for filamentous claudin-5 (Cl-5), zonula occludens-1 (ZO-1), and GLUT-1. All samples were counterstained with propidium iodide (red) to visualize cell nuclei. Scale bars = 20 μm. Images are representative of three individual experiments in triplicateBack to article page