Skip to main content
Fig. 6 | Fluids and Barriers of the CNS

Fig. 6

From: Hypoxia increases expression of selected blood–brain barrier transporters GLUT-1, P-gp, SLC7A5 and TFRC, while maintaining barrier integrity, in brain capillary endothelial monolayers

Fig. 6

Transendothelial electrical resistance (TEER) measurements and localization of HIF-1α in cells cultured in the absence and presence of 10 μM DFO under normoxic conditions or hypoxic conditions. A TEER was measured across the cell monolayers after six days of culture on permeable filter supports (i.e., experimental day). TEER is plotted as % of relative to normoxic cells without DFO (control; n = 3, total N = 18). B The mRNA expression level of SLC2A1, SLC7A5, ABCB1, CLDN5, TFRC, and VEGFR1 under normoxic and hypoxic BCECs, and cells cultured with 10 μM DFO under normoxic conditions. Expression levels were normalized relative to YHWAZ, SDHA, and HPRT-1. Data are shown as mean ± SD of three individual batches of triplicates (n = 3, total N = 9). C Immunoblots of TfR, GLUT-1, and Cl-5. D) Quantification of TfR and Cl-5 protein levels relative to the loading control β-actin. Data are representative of three individual batches (n = 3)

Back to article page

Fluids and Barriers of the CNS

ISSN: 2045-8118