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Fig. 5 | Fluids and Barriers of the CNS

Fig. 5

From: Hypoxia increases expression of selected blood–brain barrier transporters GLUT-1, P-gp, SLC7A5 and TFRC, while maintaining barrier integrity, in brain capillary endothelial monolayers

Fig. 5

Effects of DFO and YC-1 on the transendothelial electrical resistance (TEER) measurements and localization of HIF-1α. A TEER was measured across the cell monolayers cultured in the absence and presence of various concentrations of DFO or YC-1 under normoxic and hypoxic conditions on the experimental day. Measurements were normalized to the area of the filter supports. Data are shown as mean ± SD of three individual batches of two individual permeable supports (n = 3, total N = 6). B Cell monolayers were immunostained for HIF-1a (green) on day 6 of culture on Transwells and were counterstained with propidium iodide (red) to visualize cell nuclei. Cells were cultured under normoxic conditions without and with DFO, and under hypoxic concentrations without and with YC-1. The concentrations of DFO and YC-1 were 10 and 25 μM, respectively. Scale bar = 20 μm. Images are representative of three individual experiments in triplicate

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