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Fig. 4 | Fluids and Barriers of the CNS

Fig. 4

From: Hypoxia increases expression of selected blood–brain barrier transporters GLUT-1, P-gp, SLC7A5 and TFRC, while maintaining barrier integrity, in brain capillary endothelial monolayers

Fig. 4

The activities of GLUT-1, LAT-1, and P-gp in bovine brain capillary endothelial cells (BCECs) differentiated under normoxic (circles and black bars) and hypoxic (squares and grey bars) conditions. A Luminal uptake of [3H]-glucose and B) L-[4,5-3H]-leucine into BCECs was examined as a function of time. The donor concentrations of [3H]-glucose and L-[4,5-3H]-leucine was 20 and 9.5 nM, respectively. The uptake data are shown as mean ± SD of three individual cell batches of duplicates (n = 3, total N = 6). C Bidirectional transport of [3H]-digoxin across BCEC monolayers in the absence and presence of 0.4 μM zosuquidar (ZSQ). Papp values were calculated from the steady-state fluxes and determined over 120 min. The donor concentration of [3H]-digoxin was 25 nM. B-A represents transport from the basolateral to apical direction, while A–B incidates transport from apical to basolateral. Data are shown as mean ± SD of three individual batches of three individual permeable supports (n = 3, total N = 9). *p < 0.05

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Fluids and Barriers of the CNS

ISSN: 2045-8118