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Fig. 3 | Fluids and Barriers of the CNS

Fig. 3

From: Hypoxia increases expression of selected blood–brain barrier transporters GLUT-1, P-gp, SLC7A5 and TFRC, while maintaining barrier integrity, in brain capillary endothelial monolayers

Fig. 3

Transendothelial electrical resistance (TEER) measurements, mannitol permeability and immunocytochemical characterization of normoxic and hypoxic bovine brain capillary endothelial cells (BCECs). A TEER was measured across the normoxic and hypoxic cell monolayers on the day of the experiment (day 6). Red bold lines show the mean values. Measurements were normalized to the area of the filter supports (total N = 230). B The apical-to-basolateral permeability of [14C]-Mannitol was examined over a time course of 120 min. Papp values were calculated from the steady-state fluxes and determined over 120 min., and calculated from steady state fluxes. Data are shown as mean ± SD of three individual batches of three individual permeable supports (n = 3, total N = 9). *p < 0.05. C Immunocytochemical characterization of normoxic and hypoxic BCEC monolayer on day 6. The cell monolayers were immunostained for filamentous actin (F-actin), von Willebrand factor (VWF), claudin-5 (Cl-5), and zonula occludens-1 (ZO-1). All samples were counterstained with propidium iodide (red) to visualize cell nuclei. Scale bars = 50 μm. Images are representative of three individual experiments in triplicate (n = 3, total N = 9)

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Fluids and Barriers of the CNS

ISSN: 2045-8118