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Fig. 2 | Fluids and Barriers of the CNS

Fig. 2

From: Hypoxia increases expression of selected blood–brain barrier transporters GLUT-1, P-gp, SLC7A5 and TFRC, while maintaining barrier integrity, in brain capillary endothelial monolayers

Fig. 2

The gene and protein expression and localization of selected transporter proteins in bovine endothelial cells cultured in normoxic and hypoxic conditions. A Immunostaining of GLUT-1, LAT-1, P-gp, and TfR (each in green) in bovine brain capillary endothelial cells (BCECs) on Day 6 upon culture in normoxic and hypoxic conditions. Propidium iodide is red. Single or merged images are presented as XY frontal focal planes. Scale bars = 20 μm. Images are representative of three individual experiments in triplicate (n = 3, total N = 9). B Transcript levels of SLC2A1 (GLUT-1), SLC7A5 (LAT-1), ABCB1 (P-gp), and TFRC (TfR) in normoxic and hypoxic BCECs were analyzed by RT-qPCR. Expression levels were normalized to YHWAZ, SDHA, and HPRT-1. Data are shown as mean ± SD of three individual batches of triplicates (n = 3, total N = 9). C Immunoblots of GLUT-1 and P-gp in cells cultured under normoxic and hypoxic conditions, and β-actin as loading control. The left lane represents the protein ladder. D) Quantification of GLUT-1 (left) and P-gp (right) protein levels relative to β-actin. Data are representative of three individual experiments (n = 3). The mean values were compared by Student's t-test; *p < 0.05, **p < 0.005

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