Skip to main content
Fig. 1 | Fluids and Barriers of the CNS

Fig. 1

From: Hypoxia increases expression of selected blood–brain barrier transporters GLUT-1, P-gp, SLC7A5 and TFRC, while maintaining barrier integrity, in brain capillary endothelial monolayers

Fig. 1

Chronic hypoxia induces stabilization and nuclear localization of HIF-1α in bovine brain capillary endothelial cells (BCECs). A Schematic overview of the timeline for the in vitro cultivation of BCECs in normoxic and hypoxic conditions. Bovine brain microvessels were seeded onto T75 flasks on day -4 and cultivated for five days. At day 0, outgrown BCECs were passaged and seeded on permeable filter supports. The cells were cultured in cell medium (GM-) for three days in a monoculture configuration under normoxic or hypoxic conditions. The culture medium was changed to DM-TES medium on day 3, and the cells were ready for experiments on day 6. B Cell monolayers were immunostained for HIF-1α (green) after 1, 4, 12, and 72 h upon changing the medium on day 3. The samples were counterstained with propidium iodide (red) to visualize cell nuclei. Single and merged images are presented as XY frontal focal planes. Scale bar = 20 μm. Images are representative of three individual experiments in triplicate (n = 3, total N = 9)

Back to article page

Fluids and Barriers of the CNS

ISSN: 2045-8118