Modeling ischemic stroke in a triculture neurovascular unit on-a-chip

Table 2 Antibodies used for immunofluorescent staining

Primary antibodies
Antibody	Supplier	Cat. no	Dilution	Fixation	Immunogen
VE-cadherin	Abcam	ab33168	1:1000	Formaldehyde	Synthetic peptide corresponding to Human VE Cadherin aa 750 to the C-terminus conjugated to keyhole limpet haemocyanin
PECAM-1	Dako	M0823	1:20	Formaldehyde	The epitope recognized was found to be within the extracellular domain 1
ZO-1	ThermoFisher	339100	1:100	Formaldehyde	Human recombinant ZO-1 fusion protein encompassing amino acids 334–634
Claudin-5	ThermoFisher	35-2500	1:70	Methanol	Synthetic peptide derived from the mouse Claudin-5 protein
S100β	Abcam	Ab52642	1:100	Formaldehyde	Synthetic peptide within Human S100 beta aa 50 to the C-terminus (C terminal). The exact sequence is proprietary
GFAP	Abcam	Ab4674	1:1000	Formaldehyde	Recombinant full length protein corresponding to Human GFAP. Isotype 1 expressed in and purified from E. coli
B3TUBB	Novus Biologicals	NB100-1612	1:500	Formaldehyde	Chickens were immunized with synthetic peptides that corresponded to different regions of beta-III Tubulin, but are shared between the human (NP_AAL28094, NCBI) and rat (AAM28438, NCBI) protein sequences
B3TUBB	Abcam	Ab78078	1:100	Formaldehyde	Synthetic peptide corresponding to Rat beta III Tubulin aa 400–500

Secondary antibodies
Antibody	Supplier	Cat. no	Dilution	Immunogen
Goat-anti-rabbit Alexa 488+	ThermoFisher	A11008	1:250	Gamma Immunoglobins Heavy and Light chains
Goat-anti-rabbit Alexa 555	ThermoFisher	A21428	1:250	Gamma Immunoglobins Heavy and Light chains
Goat-anti-mouse Alexa 488	ThermoFisher	A11001	1:250	Gamma Immunoglobins Heavy and Light chains
Goat-anti-mouse Alexa 555+	ThermoFisher	A21422	1:250	Gamma Immunoglobins Heavy and Light chains
Goat-anti-chicken Alexa 647	ThermoFisher	A21449	1:500	Gamma Immunoglobins Heavy and Light chains
Donkey-anti-mouse Alexa 647	ThermoFisher	A31571	1:250	Gamma Immunoglobins Heavy and Light chains

ISSN: 2045-8118