Skip to main content
Fig. 4 | Fluids and Barriers of the CNS

Fig. 4

From: Modeling ischemic stroke in a triculture neurovascular unit on-a-chip

Fig. 4

Ischemic conditions cause a disruption in barrier function and reduced mitochondrial potential. The NVU model was exposed to conditions that mimic stroke for 16 h (day 14–15 of culture) by omitting glucose, addition of antimycin-A to model hypoxia, and discontinuation of flow. a Fluorescent images show representative leakage for each condition 10 min after addition of 20 kDa FITC-dextran. b Quantification of apparent permeability (Papp) of 20 kDa FITC-dextran in all conditions. Graphs show mean ± standard deviation, n = 4–5 chips. Statistical analysis was performed using Kruskal–Wallis tests for left panel graph (in which normal distribution was not confirmed) and one-way ANOVA for right panel graph; ns (not significant), *P < 0.05, **P < 0.01, ***P < 0.001. c Mitochondrial potential was assessed using a fluorescent TMRM assay in which a high fluorescent signal corresponds with healthy mitochondrial function. Fluorescent images show representative TMRM signal for each condition. d Quantification of TMRM signal in all conditions. Graphs show mean ± standard deviation, n = 4–5 chips. Statistical analysis was performed using Brown-Forsythe and Welch tests; *P < 0.05, **P < 0.01

Back to article page