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Fig. 2 | Fluids and Barriers of the CNS

Fig. 2

From: Elevated CSF inflammatory markers in patients with idiopathic normal pressure hydrocephalus do not promote NKCC1 hyperactivity in rat choroid plexus

Fig. 2

The choroid plexus remains viable ex vivo. a Calcein-AM staining of choroid plexus directly after isolation (0 h). b Calcein-AM staining of choroid plexus after 16 h of incubation in cell culture medium (16 h). c Calcein-AM staining of choroid plexus after 16 h of incubation in sterile water (16 h H2O), scale bars 500 μm. Inserts in a-c contain representative regions of the choroid plexus (white boxes) upon ×5 magnification. d 86Rb+ efflux from the choroid plexus as a function of time in control solution (ctrl, n = 5) or in presence of 20 µM bumetanide (bum, n = 5). The y-axis represents the natural logarithm of the 86Rb+ amount left in the choroid plexus at time T (AT) divided by the initial amount at time 0 (A0). Insert: 86Rb+ efflux rate constant in control solution (0.50 ± 0.10 min−1, n = 5) or in presence of 20 µM bumetanide (0.22 ± 0.06 min−1, n = 5), P = 0.0008. Error bars in d represent standard deviation and statistical significance was tested with an unpaired two-tailed t-test. ***P < 0.001

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