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Fig. 3 | Fluids and Barriers of the CNS

Fig. 3

From: Capsule-dependent impact of MAPK signalling on host cell invasion and immune response during infection of the choroid plexus epithelium by Neisseria meningitidis

Fig. 3

Inhibition of Erk1/2 and p38 signalling impacts infection rates differently in absence of the capsule. The inhibition of the Erk1/2 signalling pathway leads to an increased infection by the capsule-deficient mutants of NmB and NmC, while inhibition of p38 signalling decreases the rate of infection of the NmC capsule-deficient mutant, but has no significant effect on the infection rates of the NmB capsule-deficient mutant. HIBCPP cells were incubated for 1 h with the inhibitors U0126, SB203580 or both inhibitors (U + SB) at a concentration of 25 μM each. Untreated cells acted as controls. The HIBCPP cells were infected for 4 h at a MOI 10 in the inverted culture. The intracellular bacteria could be quantified by double immunofluorescence staining (A). The integrity of the barrier function was determined over the course of the experiment by measuring the TEER values (B) and permeability for FITC inulin (C). *** extremely significant, p < 0.001; ** highly significant, p < 0.01; * significant, p < 0.05; when comparing the infection rate of treated cells with the infection rate in the control cells

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