Fig. 1

NmB and NmC strains activate p38 strongly, while Erk1/2 is only weakly activated. NmB and NmC strains as well as their capsule-deficient mutants activate the MAPK p38 when the basolateral side of the HIBCPP cells is infected, while the MAPK Erk1/2 is only weakly activated. The activation of the MAPK Erk1/2 (A) and p38 (B) was detected by Western blotting. HIBCPP cells were infected for 4 h with NmB and NmC wild type and capsule-deficient mutants in the inverted culture model of the HIBCPP cells from the basolateral side with a MOI of 100. Untreated cells (control) served as negative controls, cells treated with PMA or Anisomycin (Aniso) acted as positive controls for the activation of the Erk1/2 or p38 signal pathways, respectively. The blots were cropped to focus on the desired bands