Fig. 8
Selective loss of tight junction proteins in leaky vessels but not angiogenic vessels. A–C, E, G Frozen brain sections taken from mice exposed to 4 days hypoxia (8% O2) were triple-labelled for CD31 (AlexaFluor-488), Ki67 (Cy3) and ZO-1 (Cy5) (A), CD31 (AlexaFluor-488), Ki67 (Cy3) and occludin (Cy5) (B), CD31 (AlexaFluor-488), IgG (Cy3) and ZO-1 (Cy5) (C), CD31 (AlexaFluor-488), IgG (Cy3) and occludin (Cy5) (E) or CD31 (AlexaFluor-488), IgG (Cy3) and claudin-5 (Cy5) (G). Scale bars = 50 μm. D, F, H Quantification of the number of ZO-1, occludin or claudin-5 positive and negative vessels after 4 days CMH. All results are expressed as the mean ± SEM (n = 4 mice/group). Note that angiogenic vessels maintained their expression of the tight junction proteins ZO-1 and occludin (A and B), but many leaky vessels lost tight junction protein expression (arrows in C, E and G)