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Fig. 7 | Fluids and Barriers of the CNS

Fig. 7

From: The impact of chronic mild hypoxia on cerebrovascular remodelling; uncoupling of angiogenesis and vascular breakdown

Fig. 7

Endothelial and microglial proliferation are associated with intravascular and extravascular patterns of fibrinogen staining, respectively. Frozen brain sections taken from mice exposed to 4 days hypoxia (8% O2) were triple-labelled for CD31 (AlexaFluor-488), BrdU (Cy3) and fibrinogen (Cy5) (A), Mac-1 (AlexaFluor-488), BrdU (Cy3) and fibrinogen (Cy5) (B), Mac-1 (AlexaFluor-488), Ki67 (Cy3) and fibrinogen (Cy5) (C), or CD31 (AlexaFluor-488), fibrinogen (Cy5) and PDGFRβ (Cy3) (D). Scale bar = 50 μm. High power images on extreme right, scale bar = 25 μm. Note that proliferating (BrdU+) endothelial cells were never associated with extravascular leak, but they were strongly associated with the intravascular fibrinogen staining pattern (arrows). In contrast, proliferating microglia were strongly associated with extravascular fibrinogen leak

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Fluids and Barriers of the CNS

ISSN: 2045-8118