Fig. 6

Examining the time course of CMH-induced endothelial proliferation and fibrinogen staining patterns. A Frozen brain sections taken from mice exposed to hypoxia (8% O₂) for 4, 7 or 14 days were stained for fibrinogen (AlexaFluor-488) and the proliferation marker Ki67 (Cy3). Scale bar = 50 μm.  Note the intravascular fibrinogen staining pattern (arrows) and the extravascular fibrinogen leaks (asterisks). B, C Quantification of the number of proliferating endothelial cells (CD31⁺/Ki67⁺ cells)/FOV (B) or vessels showing intravascular/extravascular fibrinogen staining (C) at different timepoints. All results are expressed as the mean ± SEM (n = 4 mice/group). Note the close correlation between the rate of endothelial proliferation and number of vessels showing intravascular fibrinogen staining, which both peaked at day 4 and declined thereafter. The number of vessels showing extravascular fibrinogen leak peaked at day 7 but was comparatively small at all timepoints