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Fig. 5 | Fluids and Barriers of the CNS

Fig. 5

From: The impact of chronic mild hypoxia on cerebrovascular remodelling; uncoupling of angiogenesis and vascular breakdown

Fig. 5

Analysis of intravascular fibrinogen staining. A,B Mice exposed to 4 days hypoxia (8% O2) were perfused with DiI (red channel) and frozen brain sections subsequently dual-labelled for CD31 (AlexaFluor-488) and fibrinogen (Cy5) (A), or CD31 (AlexaFluor-488) and Ki67 (Cy5) (B). C Frozen brain sections taken from mice exposed to 4 days hypoxia (8% O2) were dual-labelled for β3 integrin (Cy3) and fibrinogen (AlexaFluor-488). Scale bar = 100 μm (A, B) and 50 μm (C). Note that blood vessels showing the intravascular fibrinogen pattern or containing Ki67+ endothelial cells, stained strongly positive for DiI. Also note that while β3 integrin and fibrinogen occasionally co-localized, often they did not

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Fluids and Barriers of the CNS

ISSN: 2045-8118