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Fig. 4 | Fluids and Barriers of the CNS

Fig. 4

From: The impact of chronic mild hypoxia on cerebrovascular remodelling; uncoupling of angiogenesis and vascular breakdown

Fig. 4

Endothelial proliferation is associated with an intravascular pattern of fibrinogen staining but not with vascular leak. Frozen brain sections taken from mice exposed to 4 days hypoxia (8% O2) were triple-labelled for CD31 (AlexaFluor-488), Ki67 (Cy3) and fibrinogen (Cy5) (A–D), or laminin-111 (AlexaFluor-488), Ki67 (Cy3) and fibrinogen (Cy5) (E–F). Scale bars = 50 μm. Note that proliferating (Ki67+) endothelial cells were never associated with extravascular leak (asterisk), but they were strongly associated with an intravascular fibrinogen staining pattern (arrows). Vessels that were neither angiogenic nor leaky showed no fibrinogen staining of any kind. In panel E, also note that intravascular fibrinogen staining was found both in angiogenic laminin-111-negative capillaries (arrows) and laminin-111+ venules (arrowheads) and appeared to line the walls of blood vessels (F)

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